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Histological examination with HE and PAS staining showed glomerular cell proliferation, enlargement of the mesangial matrix, and increase in the thickness of the basement membrane, confirming the proper establishment of the DKD model (Figure 3A)

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Effects on cardiovascular morbidity and mortality In total, 33, 37 and seven RCTs reported information on (externally adjudicated) major adverse cardiovascular events (MACE), cardiovascular mortality and hospitalization due to heart failure (HHF), respectively (Supplementary Figs

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