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Researchers determine the specific conditions of each experiment, including peptide concentrations, exposure times, and study protocols, based on their research goals and institutional guidelines

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Key markers include fasting glucose and insulin (to assess insulin sensitivity), HbA1c (long-term glucose regulation), lipid panel (triglycerides, HDL, LDL), resting metabolic rate via indirect calorimetry, and body composition via DEXA scan (to differentiate fat loss from lean mass changes)

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