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After centrifugation with 18,000 g at 4C for 30 min, the supernatant was loaded on SPE columns (Phenomenex strata-X, 300 mg) that were preconditioned with 1 ml of methanol and 1 ml of water

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They found that semaglutide was linked to a 28% reduction in major adverse cardiac events (12.3% in the placebo group had such events compared to 9.1% in the semaglutide group), as well as a 24% reduction in cardiovascular disease-related deaths for this subgroup of people with pre-existing heart failure, and a 19% reduction in deaths of any cause

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