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Redox signaling is based on reversible oxidative posttranslational modifications such as thiol-disulfide switches, S-glutathionylation, and S-nitrosylation

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The chromatin was subsequently immunoprecipitated with specific antibodies (RNA poly II and H3K27ac were positive control, NIgG was the negative control) against the target protein and incubated overnight at 4 C with gentle rotation