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62,109 These examples demonstrate that LPO and ferroptosis may be biologically irrelevant for APAP hepatotoxicity under normal conditions because endogenous defense mechanisms (such as the lipid-soluble antioxidant vitamin E and the iron chelator ferritin) effectively prevent excessive LPO

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Fixed cells were then washed three times with PBS and then permeabilized in 500 L 0.1% Triton X-100 in PBS for 10 min

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