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57% were cisgender men, 37% cisgender women, and 6% transgender women

After manual removal of contaminants by comparison with the blank samples, GC-MS data were first normalised by the multiple internal standards (2,3,3,3-d4-alanine, 2,3,4,5,6-d5-phenylalanine, 3,3-d2-tyrosine) and initial batch correction was performed using median centring according to metabolite levels in the QC samples

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