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Step 2 - Apply 3-5 drops of the serum on the entire face and neck

Western blot Cells were lysed in 1 RIPA buffer (Thermo Scientific, 89900) supplemented with cOmplete mini EDTA-free protease inhibitor tablets (Roche, 04693159001) and PhosSTOP phosphatase inhibitor tablets (Roche, 04906837001) and incubated on ice for 10 min

GAPDH, glyceraldehyde 3 phosphate dehydrogenase

We compared the vascular permeability on the non-ligature side and on the ligature side in each of the three groups

By integrating multiple algorithms, researchers can leverage the strengths of different models to further improve diagnostic precision and reliability [196, 197]